Setting up a non-invasive LC-MS platform for semi-quantification of steroid hormones in anurans
Setting up a non-invasive LC-MS platform for semi-quantification of steroid hormones in anurans
Samenvatting
Amphibians, and in particular anurans, are amongst the most endangered species on earth. Comparing the physiology between urban and rural anurans provides key information on the effect of urbanization on anurans. In turn, this information can help with preservation and conservation of
the species as a whole. The goal of this study was to set up a liquid chromatograhy-mass spectrometry platform, including sampling, sample preparation and analysis methods, that can be used to learn more about impact of urbanization on the physiology of anurans. The steroid hormones testosterone and corticosterone were chosen as main analytes for this study, because there is a high probability they are measurably excreted by anurans. The sampling method on which this study was based utilized water-bath spotting on dried filter papers to sample anurans non-invasively and with relative ease. A complementary sample preparation method and an analysis method on a liquid chromatography-mass spectrometry system were set up and validated. A strong foundation was laid out to implement the platform in the future on real world samples following minor adjustments.
Organisatie | Hogeschool Leiden |
Opleiding | Chemie |
Afdeling | Faculteit Techniek |
Datum | 2020-11-05 |
Type | Bachelor |
Taal | Engels |