Samenvatting

Drought, caused by climate change, poses a significant threat to the production of barley. To ensure high yields, drought adaptation in barley is critical. Roots are primarily responsible for water uptake, and during drought, when water levels are low, deep rooting traits are crucial for developing drought-tolerant crops. However, roots are difficult to study due to their location and therefore a
different phenotyping technique using above-ground traits is essential to determine the root system architecture (RSA) of barley during drought. In this study, the relationship between canopy temperature (CT) and rooting depth is explored through thermography. A controlled lysimeter experiment with four genotypes under both drought and well-watered conditions, as well as the
results of a large-scale rainfed field trial with 20 genotypes, were used to grow and measure the CT of barley across drought, well-watered, and wet environments. The lysimeter experiment revealed significant differences in CT between climatic treatments (P