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Preparation of hollow nano-capsules via RAFT polymerization by vesicle-template method

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Preparation of hollow nano-capsules via RAFT polymerization by vesicle-template method

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Samenvatting

This report is about the synthesis of polymer nanocapsules, to be used in
targeted drug delivery. The ultimate goal is to synthesize temperature
responsive polymer nanocapsules which can be administrated intravenous
by human tissue and thus have to be smaller than 200 nm. To synthesize
these nanocapsules a simple RAFT-based vesicle templating approach is
used. In the first step the vesicles are prepared by extruding a
dioctadecyldimethylammonium bromide (DODAB) solution through filters
with 100 nm or 200 nm pores. During the polymerization the premade
RAFT-oligomer was attached to the vesicles after which the monomers
were added. The gained capsules were analyzed by dynamic light
scattering (DLS) to measure the size, by gel permeation chromatography
(GPC) to measure the molecular weight, scanning electron microscopy
(SEM) and transmission electron microscopy (TEM) were used to give
information on shape and morphology of the capsules.
The parameters varied to optimize the results was the ratio of the
monomers methyl methacrylate (MMA), tert-butyl acrylate (t-BA) and
ethylene glycol dimethyl acrylate (EGDMA) and the ratio of the monomers
N-isopropylacrylamide (NIPAm), N, N-methylenebisacrylamide (MBA) and
acrylic acid (AA). Both vesicles gained with the 200 nm filters and the 100
nm filters were used during this research. The average diameter found
with DLS was 190 nm for the capsules made with the vesicles that were
extruded through the 200 nm filters. An average of 133 nm was found for
the capsules that were made with vesicles that were extruded through the
100 nm filters. The synthesized polymer capsules are reproducible. The
capsules made with NIPAm, MBA and AA show size change due to
temperature differences. This means that temperature responsive
capsules can be made. Unfortunately the size change caused by the
temperature difference between healthy and diseased human tissue is
only 0.3 nm, which not enough for targeted drug delivery. In future
research different copolymers could be tested to change the large peak to
the biologically critical temperature of diseased tissue. At last a spherical
shape of the capsules was found with TEM analysis as expected.

Toon meer
OrganisatieAvans Hogeschool
OpleidingChemie-Breda
AfdelingATGM Academie voor de technologie van Gezondheid en Milieu
PartnersTechnische Universiteit Eindhoven
Datum2013-06-13
TypeBachelor
TaalEngels

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