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A Lipooligosaccharide Silencing RNA regulates Campylobacter jejuni pathogenicity

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A Lipooligosaccharide Silencing RNA regulates Campylobacter jejuni pathogenicity

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Samenvatting

The bacterial cell envelope is the most important structure to counteract the early stages of (a)biotic stressors. Bacteriophages and transcytosis across epithelial cells both induce stress on the bacterial cell envelope. In Campylobacter jejuni, modification of the bacterial cell envelope and Clusters of Regulatory Interspaced Short Palindromic Repeats and associated genes (CRISPR-Cas) have both been linked to defense and virulence features. Current evidence suggests that a dual function exist between regulation of cell envelope exposed Sialic Acid-containing Carbohydrates (SACS) and the CRISPR-Cas system in C. jejuni. Presence of SACS makes C. jejuni isolates highly pathogenic, provoke severe colitis and induce post-infectious complications in susceptible patients. Lack of SACS or the CRISPR-Cas marker gene cas9 strongly altered C. jejuni transcytosis across polarized Caco-2 cells, the ability to cause cellular damage and susceptibility to viruses. Recently, the Cas9 nuclease and two small CRISPR associated RNAs in Francisella novicida have been shown to regulate the lipoprotein expression on the cell envelope that is involved in cell host entrance and immune recognition. In C. jejuni, these two small CRISPR RNAs might not only control the lipoprotein expression, but also the cell envelope exposed SACS during viral defense and virulence features. Indeed, our results indicate that C. jejuni uses its CRISPR-Cas system to regulate its cell envelope exposed SACS in order to survive encountered biotic stresses in the environment or inside a host.

Toon meer
OrganisatieAvans Hogeschool
OpleidingBiologie en Medisch Laboratoriumonderzoek-Breda
AfdelingATGM Academie voor de technologie van Gezondheid en Milieu
PartnersErasmusMC
Datum2014-06-03
TypeBachelor
TaalEngels

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